Prestorage leukoreduced RBCs: Evaluation for two different quadruple collection bags with an inline RBC filter / 대한수혈학회지

ABSTRACT

BACKGROUND: The Korean Red Cross(KRC) are considering the introduction of prestorage leukoreduced RBCs, as it is thought one of best way to prevent WBC-associated adverse transfusion reactions. We evaluated two different quadruple top-and-bottom collection bags, specially designed for this purpose by two manufacturers(Boin Medica Co.,Ltd and Green Cross Medical Corporation, Korea), consisted of a CPD bag, a SAG-M bag and an inline RBC filter, either RCM1 (Pall Medical, U.S.A.) or BioR 01 1N (Fresenius Hemocare, Germany) each. METHODS: Fifty one bags from Boin and sixty bags from Green Cross and consequent blood components were studied in twice over a period of two years. As an inline filter, Boin used RCM1 and Green Cross used RCM1(1st) and BioR 01 1N(2nd). They were divided into group A(filtration performed after processing of RBCs) and B(filtration performed after storage of overnight at 4 degrees C). WBC-reduced RBCs were tested for pH, plasma K+, plasma Hb and blood culture every 7 days to investigate storage time-dependent changes up to 42 days. RESULTS: The results of pH, plasma K+ and plasma Hb from CPD added whole blood, RBCs, SAG-M added RBCs and WBC-reduced RBCs did not show significant differences. We observed both of adding SAG-M solution and filtration procedure did not effect on the prestorage leukoreduced RBCs. WBCs were removed effectively by prestorage filtration. But in the case of failed units, the design or method to integrate an RBC filter may be a cause for the failure. CONCLUSION: We concluded that two different quadruple collection bags with integral RCM1 filter from Boin(2nd) and Green Cross(1st) are useful for production of prestorage leukoreduced RBCs.