The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells / 대한치과보존학회지
Journal of Korean Academy of Conservative Dentistry
;
: 356-363, 2009.
Article
in Korean
| WPRIM
| ID: wpr-125392
ABSTRACT
The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4degrees C for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37degrees C water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontal Ligament
/
Tiletamine
/
Tooth
/
Baths
/
Dimethyl Sulfoxide
/
Eosine Yellowish-(YS)
/
Freezing
/
Anesthesia
/
Molar
Limits:
Animals
/
Female
/
Humans
Language:
Korean
Journal:
Journal of Korean Academy of Conservative Dentistry
Year:
2009
Type:
Article
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