The Role of Insulin-like Growth Factor I(IGF-I), and IGF Binding Protein (IGFBP) in Mouse Lung Cancer Cells / 결핵
Tuberculosis and Respiratory Diseases
;
: 549-556, 2001.
Article
in Korean
| WPRIM
| ID: wpr-125530
ABSTRACT
BACKGROUND:
IGF-I is an important mitogen in many types of malignancies. Tumors also express many IGF binding proteins, which modulate IGF action. The purpose of this study was to evaluaste the effect of IGF-I and IGFBP on cell proliferation in mouse lung cancer cells (3LL).METHODS:
The cellular proliferation of 3LL with the treatment of growth factors was evaluated using MTT assay. Western ligand blot was performed in order to determine whether 3LL cells secrete IGFBPs and we evaluated the effect of IGFBP on cellular proliferation.RESULTS:
The treatment of 3LL cells with IGF-I increased cellular proliferation in a serum free media. Western ligand blot of conditioned medium of 3LL with 125I-IGF-I demonstrated one single major band with an estimated molecular mass of 24 kDa. This band was identified as IGFBP-4 with immunoblot analysis using antisera. The addition of anti-IGFBP-4 antibody to abrogate the effect of IGFBP-4 resulted in increased cellular prolife ration suggesting that IGFBP-4 inhibits cell growth.CONCLUSION:
IGF-I increases cellular proliferation, however the secreted IGFBP- 4 has an ingibitory function on cell growth in 3LL. These findings suggest that IGF-I and IGFBP are involved in the cell proliferation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Insulin-Like Growth Factor I
/
Carrier Proteins
/
Culture Media, Serum-Free
/
Culture Media, Conditioned
/
Insulin-Like Growth Factor Binding Proteins
/
Insulin-Like Growth Factor Binding Protein 4
/
Intercellular Signaling Peptides and Proteins
/
Cell Proliferation
/
Immune Sera
/
Lung
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Tuberculosis and Respiratory Diseases
Year:
2001
Type:
Article
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