Usage of T Cell receptor repertoire is restricted in synovial lymphocytes in rheumatoid arthritis
Immune Network
;
: 70-76, 2001.
Article
in Korean
| WPRIM
| ID: wpr-125535
ABSTRACT
BACKGROUND:
Rheumatoid arthritis is an autoimmune disease characterized by a chronic inflammatory process, primarily involving the synovial membrane of peripheral joints, where T cell activation is found. To address the superantigen stimulation in rheumatoid arthritis, T cell clonality and the expression of activation markers were analyzed.METHODS:
To detect TCRB V usage, inverse PCR and sequencing were done. Monoclonal antibodies were used for flow cytometric analysis of TCRBV8 or TCRBV5. As result s, a restricted usage of TCRBV3 gene was detected in synovial lymphocytes from one rheumatoid arthritis patient . However, preferential usage for TCRB V8, which may be one indicator for stimulation by staphylococcal superantigen, was not obvious although general activation of T cells was found as high DR+ percentage in synovial T cells. These data show specific antigen rather than superantigen might involve the pathogenesis of rheumatoid arthritis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arthritis, Rheumatoid
/
Autoimmune Diseases
/
Synovial Membrane
/
Lymphocytes
/
Receptors, Antigen, T-Cell
/
T-Lymphocytes
/
Polymerase Chain Reaction
/
Joints
/
Antibodies, Monoclonal
Limits:
Humans
Language:
Korean
Journal:
Immune Network
Year:
2001
Type:
Article
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