Two A(weak)B Cases with Aw10 Allele Separated by Allele-specific Sequencing / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 59-64, 2011.
Article
in Ko
| WPRIM
| ID: wpr-125624
Responsible library:
WPRO
ABSTRACT
We separated an Aw10 allele by allele-specific sequencing in two Aw10/B101 samples that had the AweakB phenotype. Two samples with the A102/B101 genotype were also tested as a control. The reverse primers using position 930 at exon7 were designed for allele-specific sequencing. The differential positions were a total of 52 points for distinguishing the A-allele from the B-allele. Although overlaps with another haplotype allele that showed a minor chromatographic peak were observed in almost all the points, the specific allele-separation rate was 100% (52/52) by assessing the dominance in the chromatographic peak height. Based on the separation rate in the two cases with Aw10/B101 and the two AB controls, allele-specific sequencing is a convenient and reliable method for the separating the A-allele and B-allele in a clinical laboratory.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Haplotypes
/
Alleles
/
Genotype
Language:
Ko
Journal:
Korean Journal of Blood Transfusion
Year:
2011
Type:
Article