Carnosic Acid Inhibits Lipid Accumulation in 3T3-L1 Adipocytes Through Attenuation of Fatty Acid Desaturation
Journal of Cancer Prevention
;
: 41-49, 2015.
Article
in English
| WPRIM
| ID: wpr-125852
ABSTRACT
BACKGROUND:
Excess body fat accumulation contributes to the development of metabolic disorders that can cause adverse health effects. Carnosic acid (CA), a major bioactive component of rosemary (Rosemarinus officinalis), has been suggested to possess anti-adipogenic properties. The present study was conducted to elucidate the mechanism underlying the anti-adipogenic effects of CA.METHODS:
3T3-L1 pre-adipocytes were treated with CA (0.1, 1, and 10 muM) from day 0 to day 8 of differentiation. On day 8, biochemical markers of lipid accumulation and the degree of fatty acid desaturation were measured.RESULTS:
Oil Red O staining results, triglyceride (TG) accumulation, and glycerol 3-phosphate dehydrogenase activity suggested that CA significantly inhibited lipid accumulation in 3T3-L1 adipocytes. CA significantly decreased mRNA expression of peroxisome proliferator-activated receptor-gamma, sterol regulatory element-binding protein 1, and CCAAT/enhancer binding protein-alpha in a dose-dependent manner. Moreover, it decreased the ratio of both C161/C160 and C181/C180, with reduced expression of stearoyl CoA desaturase 1 mRNA and protein.CONCLUSIONS:
These results suggest that CA efficiently suppressed adipogenesis in 3T3-L1 adipocytes and its action, at least in part, is associated with the downregulation of adipogenesis-related genes and the fatty acid composition of TG accumulated in adipocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidoreductases
/
Stearoyl-CoA Desaturase
/
Triglycerides
/
RNA, Messenger
/
Biomarkers
/
Down-Regulation
/
Adipose Tissue
/
Adipocytes
/
Peroxisomes
/
Sterol Regulatory Element Binding Protein 1
Language:
English
Journal:
Journal of Cancer Prevention
Year:
2015
Type:
Article
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