Your browser doesn't support javascript.
loading
miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1
Yonsei Medical Journal ; : 234-240, 2017.
Article in English | WPRIM | ID: wpr-126252
ABSTRACT

PURPOSE:

MicroRNAs are small non-coding RNAs that play important roles in vascular smooth muscle cell (VSMC) function. This study investigated the role of miR-379 on proliferation, invasion, and migration of VSMCs and explored underlying mechanisms thereof. MATERIALS AND

METHODS:

MicroRNA, mRNA, and protein levels were determined by quantitative real-time PCR and western blot. The proliferative, invasive, and migratory abilities of VSMCs were measured by CCK-8, invasion, and wound healing assay, respectively. Luciferase reporter assay was used to confirm the target of miR-379.

RESULTS:

Platelet-derived growth factor-bb was found to promote cell proliferation and suppress miR-379 expression in VSMCs. Functional assays demonstrated that miR-379 inhibited cell proliferation, cell invasion, and migration. Flow cytometry results further showed that miR-379 induced apoptosis in VSMCs. TargetScan analysis and luciferase report assay confirmed that insulin-like growth factor-1 (IGF-1) 3'UTR is a direct target of miR-379, and mRNA and protein levels of miR-379 and IGF-1 were inversely correlated. Rescue experiments showed that enforced expression of IGF-1 sufficiently overcomes the inhibitory effect of miR-379 on cell proliferation, invasion, and migration in VSMCs.

CONCLUSION:

Our results suggest that miR-379 plays an important role in regulating VSMCs proliferation, invasion, and migration by targeting IGF-1.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Sincalide / Wound Healing / RNA, Messenger / Insulin-Like Growth Factor I / Cell Movement / Apoptosis / Proto-Oncogene Proteins c-sis / MicroRNAs / Cell Proliferation / Real-Time Polymerase Chain Reaction Limits: Humans Language: English Journal: Yonsei Medical Journal Year: 2017 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Sincalide / Wound Healing / RNA, Messenger / Insulin-Like Growth Factor I / Cell Movement / Apoptosis / Proto-Oncogene Proteins c-sis / MicroRNAs / Cell Proliferation / Real-Time Polymerase Chain Reaction Limits: Humans Language: English Journal: Yonsei Medical Journal Year: 2017 Type: Article