miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1
Yonsei Medical Journal
;
: 234-240, 2017.
Article
in English
| WPRIM
| ID: wpr-126252
ABSTRACT
PURPOSE:
MicroRNAs are small non-coding RNAs that play important roles in vascular smooth muscle cell (VSMC) function. This study investigated the role of miR-379 on proliferation, invasion, and migration of VSMCs and explored underlying mechanisms thereof. MATERIALS ANDMETHODS:
MicroRNA, mRNA, and protein levels were determined by quantitative real-time PCR and western blot. The proliferative, invasive, and migratory abilities of VSMCs were measured by CCK-8, invasion, and wound healing assay, respectively. Luciferase reporter assay was used to confirm the target of miR-379.RESULTS:
Platelet-derived growth factor-bb was found to promote cell proliferation and suppress miR-379 expression in VSMCs. Functional assays demonstrated that miR-379 inhibited cell proliferation, cell invasion, and migration. Flow cytometry results further showed that miR-379 induced apoptosis in VSMCs. TargetScan analysis and luciferase report assay confirmed that insulin-like growth factor-1 (IGF-1) 3'UTR is a direct target of miR-379, and mRNA and protein levels of miR-379 and IGF-1 were inversely correlated. Rescue experiments showed that enforced expression of IGF-1 sufficiently overcomes the inhibitory effect of miR-379 on cell proliferation, invasion, and migration in VSMCs.CONCLUSION:
Our results suggest that miR-379 plays an important role in regulating VSMCs proliferation, invasion, and migration by targeting IGF-1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sincalide
/
Wound Healing
/
RNA, Messenger
/
Insulin-Like Growth Factor I
/
Cell Movement
/
Apoptosis
/
Proto-Oncogene Proteins c-sis
/
MicroRNAs
/
Cell Proliferation
/
Real-Time Polymerase Chain Reaction
Limits:
Humans
Language:
English
Journal:
Yonsei Medical Journal
Year:
2017
Type:
Article
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