Reconstruction of Artificial Skin In Vitro / 체질인류학회지

ABSTRACT

Artificial skin can be employed as a useful model for investigating skin disease and clinical application such as burn. To evaluate this, the skin was reconstructed by culturing keratinocytes in airfluid interface over the collagen lattice and ultrastructures was also investigated by electron microscope. Epidermis showed more than 10 layers including basal, spinous, granular and honey layers but basement membrane and anchoring fibrils were not formed at the dermoepidermal junction. Desmosomes were formed at cell junctions but reduced in number. Intermediate filaments were scarce and fine, especially at the spinosal layer. Keratohyalin granules were smaller and fewer than those of in vivo. Parakeratosis was observed at the honey layer. It was, therefore, concluded that the artificial skin could be a valuable experimental model although the differentiation process of keratinocytes was somewhat unstable.