Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE
Journal of Veterinary Science
;
: 65-73, 2007.
Article
in English
| WPRIM
| ID: wpr-126336
ABSTRACT
The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6 distinct fingerprint patterns, respectively. The most common identical RAPD pattern was produced by OPA-07 which was shared by 32 isolates (71.1%), the pattern produced by OPA-08 was shared by 26 isolates (57.8%), and the pattern produced by OPA-18 was shared by 40 isolates (88.9%). The RAPD patterns of the vaccine strain and the 3 reference strains did not match any of the patterns produced by the field isolates when primers OPA-07 and OPA-08 were used. PFGE using the restriction endonuclease XbaI produced a total of 15 patterns consisting of 4 PFGE types (A, B, B1 and C, differing by > or = 4 bands) and 11 A subtypes (differing by < or = 3 bands). Most of the field isolates exhibited identical type A and B patterns, suggesting that they were related. The vaccine strain and the three reference strains showed different PFGE patterns as compared to the identical type A strains.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Species Specificity
/
Swine
/
Genetic Variation
/
Cluster Analysis
/
Electrophoresis, Gel, Pulsed-Field
/
Bordetella bronchiseptica
/
DNA Primers
/
Random Amplified Polymorphic DNA Technique
/
Korea
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Veterinary Science
Year:
2007
Type:
Article
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