Enhanced Atrial Natriuretic Peptide and Nitric Oxide System Following the Treatment with Caffeine in Rats / 대한신장학회잡지
Korean Journal of Nephrology
;
: 123-128, 2002.
Article
in Korean
| WPRIM
| ID: wpr-126469
ABSTRACT
PURPOSE:
The present study was aimed to examine whether caffeine alters the local regulation of atrial natriuretic peptide(ANP) and nitric oxide(NO) systems in the kidney.METHODS:
Male Sprague-Dawley rats were treated with caffeine, consisting of a single oral bolus(0.2%, 1 mL/kg) followed by supplementation in drinking water(0.2%) for 1 day. Rats treated the same without caffeine served as control. The tissue expression of ANP mRNA was determined by reverse transcription-polymerase chain reaction. Tissue levels of nitrite/nitrate were determined. The expression of aquaporin(AQP)-1 and AQP2 proteins was determined by Western blot analysis.RESULTS:
Following the treatment with caffeine, the expression of ANP mRNA was increased in the kidney. The renal tissue nitrite/nitrate level was also increased by caffeine-treatment. On the other hand, the expression of AQP1 and AQP2 proteins was not significantly altered.CONCLUSION:
The enhanced activities of local ANP and NO systems may in part be causally related with the caffeine-induced natriuresis and diuresis, while AQP channels are not involved.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Caffeine
/
RNA, Messenger
/
Blotting, Western
/
Atrial Natriuretic Factor
/
Rats, Sprague-Dawley
/
Diuresis
/
Drinking
/
Aquaporin 2
/
Hand
/
Kidney
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Nephrology
Year:
2002
Type:
Article
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