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Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice / 한국실험동물학회지
Laboratory Animal Research ; : 14-20, 2014.
Article in English | WPRIM | ID: wpr-126817
ABSTRACT
Bone marrow (BM) has been considered as a reservoir of stem/progenitor cells which are able to differentiate into ectodermal, endodermal, and mesodermal origins in vitro as well as in vivo. Following adequate stimulation, such as granulocyte stimulating factor (G-CSF) or AMD3100, BM resident stem/progenitor cells (BMSPCs) can be mobilized to peripheral blood. Several host-related factors are known to participate in this mobilization process. In fact, a significant number of donors are resistant to G-CSF induced mobilization protocols. AMD3100 is currently used in combination with G-CSF. However, information regarding host-related factors which may influence the AMD3100 directed mobilization is extremely limited. In this study, we were to get some more knowledge on the host-related factors that affect the efficiency of AMD3100 induced mobilization by employing in vivo mobilization experiments. As a result, we found that C57BL/6J mice are more sensitive to AMD3100 but less sensitive to G-CSF which promotes the proliferation of BMSPCs. We excluded S1P as one of the host related factor which influences AMD3100 directed mobilization because pre-treatment of S1P receptor antagonist FTY720 did not inhibit BMSPC mobilization. Further in vitro experiments revealed that BALB/c mice, compared to C57BL/6J mice, have less BMSPCs which migrate in response to host related factors such as sphingosine-1-phosphate (S1P) and to CXCL12. We conclude that AMD3100-directed mobilization depends on the number of BMSPCs rather than on the host-related factors. These results suggest that the combination of AMD3100 and G-CSF is co-operative and is optimal for the mobilization of BMSPCs.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Tissue Donors / Bone Marrow / Granulocyte Colony-Stimulating Factor / Receptors, Lysosphingolipid / Ectoderm / Endoderm / Fingolimod Hydrochloride / Granulocytes / Mesoderm Type of study: Practice guideline Limits: Animals / Humans Language: English Journal: Laboratory Animal Research Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Tissue Donors / Bone Marrow / Granulocyte Colony-Stimulating Factor / Receptors, Lysosphingolipid / Ectoderm / Endoderm / Fingolimod Hydrochloride / Granulocytes / Mesoderm Type of study: Practice guideline Limits: Animals / Humans Language: English Journal: Laboratory Animal Research Year: 2014 Type: Article