Ferulic acid prevents the injury-induced decrease of gamma-enolase expression in brain tissue and HT22 cells / 한국실험동물학회지
Laboratory Animal Research
;
: 8-13, 2014.
Article
in English
| WPRIM
| ID: wpr-126818
ABSTRACT
Ferulic acid is known to act as a protective agent in cerebral ischemia through its anti-oxidant activity. gamma-Enolase is a neuron-specific enolase that also exerts a neuroprotective effect. Here, we investigated whether ferulic acid regulates the expression level of gamma-enolase in middle cerebral artery occlusion (MCAO)-induced brain injury and glutamate exposure-induced neuronal cell death. Adult male rats were treated with either vehicle or ferulic acid (100 mg/kg, i.v.) after MCAO and cerebral cortex tissues were collected 24 h after MCAO. Using a proteomics approach, we found that gamma-enolase expression was decreased in MCAO-injured animals treated with vehicle alone, whereas ferulic acid treatment attenuated this decrease. Reverse-transcription PCR and Western blot analyses confirmed that ferulic acid treatment prevented MCAO injury-induced decrease in gamma-enolase. Furthermore, in hippocampal-derived cell lines, glutamate exposure also decreased gamma-enolase expression and ferulic acid treatment attenuated this glutamate-induced decrease in gamma-enolase. These findings suggest that ferulic acid mediates a neuroprotective effect by attenuating injury-induced decreases of gamma-enolase expression in neuronal cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphopyruvate Hydratase
/
Brain
/
Brain Injuries
/
Cell Line
/
Cerebral Cortex
/
Brain Ischemia
/
Blotting, Western
/
Polymerase Chain Reaction
/
Cell Death
/
Neuroprotective Agents
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Laboratory Animal Research
Year:
2014
Type:
Article
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