Effects of 2-deoxy-D-glucose and quercetin on cytokine secretion and gene expression of type I collagen during osteoblastic differentiation in irradiated MC3T3-E1 cells / 대한구강악안면방사선학회지

ABSTRACT

PURPOSE: To characterize the effects of 2-deoxy-D-glucose (2DG) and quercetin (QCT) on cytokine secretion of IL-6, TGF-beta and gene expression of Col I in irradiated MC3T3-E1 cells. MATERIALS AND METHODS: The MC3T3-E1 cells were cultured in an alpha-MEM supplemented with 5 mM 2DG or 10 mM QCT and then the cells were incubated 12h before irradiation with 2, 4, 6, and 8 Gy X-ray using a linear accelerator delivered at a dose rate of 1.5 Gy/min. Level of IL-6 and TGF-beta was determined by ELISA. Also expression of Col I was examined by RT-PCR. RESULTS: In accordance with the radiation dose, the amount of TGF-beta was not different in RA+QCT, but it showed a peak value in control and RA+2DG at 4Gy on the 3rd day. However, all groups showed a decreasing tendency dose-dependently in RA+QCT on the 7th day (p< 0.01). In accordance with the radiation dose, the amount of IL-6 increased dose-dependently in all groups on the 3rd day. On the 7th and 21st day, all groups showed peak values at 4Gy. RA+QCT showed a slightly increased amount of IL-6 at 2 Gy, but it showed a slightly decreased amount at 4, 6, and 8 Gy. In accordance with the period of culture after irradiation, the expression of Col I increased dosedependently in RA+QCT. CONCLUSIONS: The result showed that QCT acted as radiosensitizer in the secretion of TGF-beta and gene expression of Col I during differentiation in irradiated MC3T3-E1 cells at the cellular level.