Preparation and Characterization of SDF-1alpha Recombinant Adenovirus for ex vivo Expansion of Hematopoietic Stem Cells
Journal of Bacteriology and Virology
;
: 39-47, 2005.
Article
in Korean
| WPRIM
| ID: wpr-127010
ABSTRACT
Stromal cell-derived factor 1 (SDF-1/CXCL12) is a multifunctional cytokine implicated in normal hematopoiesis. We previously reported that SDF-1alpha enhanced the survival of hematopoietic stem and progenitor cells in synergy with other cytokine such as GM-CSF, steel factor, or thrombopoietin. As adult stem cells are very rare, many investigators are trying to expand hematopoietic stem/progenitor cells in vitro. In this study, we constructed an adenoviral vector and produced high titer of recombinant adenoviruses directing robust expression of SDF-1alpha determined by ELISA. We also produced control empty adenoviruses and recombinant LacZ adenoviruses. In order to check the feasibility of SDF-1alpha in ex vivo expansion system, we compared HUVEC cells tranduced by a SDF-1alpha recombinant virus with HUVEC cells transduced by a LacZ recombinant virus in supporting activity of hematopoietic cells, and found that expression of SDF-1alpha in HUVEC cells increased viable blood cell population obtained from the same number of CD34+ cells. The SDF-1alpha recombinant adenovirus seems to be useful for future application in hematopoiesis studies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Research Personnel
/
Stem Cells
/
Thrombopoietin
/
Blood Cells
/
Enzyme-Linked Immunosorbent Assay
/
Hematopoietic Stem Cells
/
Adenoviridae
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Stem Cell Factor
/
Adult Stem Cells
Limits:
Humans
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2005
Type:
Article
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