Hypermethylation of Tumor-related Genes in Genitourinary Cancer Cell Lines
Journal of Korean Medical Science
;
: 756-761, 2001.
Article
in English
| WPRIM
| ID: wpr-127189
ABSTRACT
Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostatic Neoplasms
/
Urinary Bladder Neoplasms
/
Tumor Cells, Cultured
/
Cadherins
/
Genetic Testing
/
Polymerase Chain Reaction
/
Urogenital Neoplasms
/
DNA Primers
/
DNA Methylation
/
O(6)-Methylguanine-DNA Methyltransferase
Limits:
Humans
/
Male
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2001
Type:
Article
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