Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction
Journal of Veterinary Science
;
: 231-237, 2005.
Article
in English
| WPRIM
| ID: wpr-128173
ABSTRACT
A multiplex PCR assay was developed for the simultaneous detection of the etiologic agents associated with porcine proliferative enteropathies (PPE), swine dysentery (SD)and porcine salmonellosis (PS)in a single reaction using DNA from swine intestinal samples. Single and multiplex PCR amplification of DNA from Lawsonia intracellularis, Salmonella typhimurium and Brachyspira hyodysenteriae with each primer set produced fragments of the predicted size without any nonspecific amplification, 210-bp, 298-bp and 403-bp bands, respectively. The single PCR assay could detect as little as 100 pg of purified DNA of S. typhimurium and L. intracellularis, and 50 pg of B.hyodysenteriae, respectively. However, multiplex PCR turned out to be 10 times lower sensitivity with S. typhimurium compared with single PCR. With 23 swine intestinal specimens suspected of having PPE, SD and/or PS, the multiplex PCR assay showed identical results with conventional methods except one. In conclusion, this multiplex PCR is a feasible alternative to standard diagnostic methods for detection of L. intracellularis, B. hyodysenteriae and Salmonella spp. from swine intestinal specimens.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Salmonella
/
Salmonella Infections, Animal
/
Spirochaetales
/
Spirochaetales Infections
/
Swine
/
Swine Diseases
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Lawsonia Bacteria
/
Desulfovibrionaceae Infections
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2005
Type:
Article
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