Development of Rickettsia Specific Nested PCR Method Based on groEL Gene Sequences
Journal of Bacteriology and Virology
;
: 301-306, 2003.
Article
in Korean
| WPRIM
| ID: wpr-128193
ABSTRACT
To detect Rickettsia, we have developed a nested PCR method amplifying the groEL gene. Rickettsia strains were successfully amplified by this PCR method but the microorganisms causing other febrile diseases, such as Orientia tsutsugamushi, Coxiella burnetii, Ehrlichia sennetsu, Borrelia burgdorferi sensu lato, Borrelia hermsii, and Leptospira interrogans were not amplified. This PCR assay was applied to detect Rickettsia DNA from 100 ticks. Sixteen Haemaphysalis longicornis ticks were positive by this PCR assay. These results suggest that the new nested PCR method might be sensitive and useful for discrimination between Rickettsia and other febrile disease-causing microorganisms.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rickettsia
/
Orientia tsutsugamushi
/
Ticks
/
Borrelia
/
DNA
/
Borrelia burgdorferi Group
/
Polymerase Chain Reaction
/
Coxiella burnetii
/
Neorickettsia sennetsu
/
Discrimination, Psychological
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2003
Type:
Article
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