Evaluation of expression patterns of feline CD28 and CTLA-4 in feline immunodeficiency virus (FIV)-infected and FIV antigen-induced PBMC
Journal of Veterinary Science
;
: 97-103, 2000.
Article
in English
| WPRIM
| ID: wpr-128399
ABSTRACT
It is known that CD28, a positive costimulatory receptor, plays a very important role in inducing the optimal stimulation of T lymphocytes. CTLA-4 (CD152), however, acts as a negative regulator in T lymphocyte activation. The effect of an feline immunodeficiency virus (FIV) infection on the expression of feline CD28 and CTLA-4 was studied with FIV-infected and uninfected peripheral blood mononuclear cells (PBMC) using a competitive PCR assay. The nature of CD28 and CTLA-4 expression was also examined with fresh and antigen-stimulated PBMC. FIV infection induced a lower expression of CD28, but a higher expression of CTLA-4 in the infected PBMC than in the uninfected PBMC. Relatively high levels of CD28 expression were demonstrated in both the fresh and the antigen-stimulated PBMC. The expression level of CTLA-4 in the freshly isolated PBMC was rather low, however, FIV antigen stimulation induced a relatively high expression of CTLA-4 in feline PBMC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukocytes, Mononuclear
/
Antigens, Differentiation
/
Antigens, CD
/
Gene Expression
/
Cell Survival
/
Cells, Cultured
/
Polymerase Chain Reaction
/
CD28 Antigens
/
Immunodeficiency Virus, Feline
/
Antigens, Viral
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2000
Type:
Article
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