Immunologic reactivity of a lipopolysaccharide-protein complex of type A Pasteurella multocida in mice
Journal of Veterinary Science
;
: 87-95, 2000.
Article
in English
| WPRIM
| ID: wpr-128400
ABSTRACT
The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were mitogenic for mouse B lymphocytes with the protein moiety the most active. Although immune serum against the LPS-protein complex protected mice against challenge thereby indicating a role for humoral immunity, the LPS-protein complex of P. multocida was also found to induce cell-mediated immunity. This cell-mediated immunity was demonstrated in mice immunized with the complex by (1). mitogenic responses of T lymphocytes, (2). induction of delayed type hypersensitivity reaction in the hind footpads, and (3). enhanced resistance to challenge infection with Salmonella enteritidis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pasteurella Infections
/
Salmonella enteritidis
/
Salmonella Infections, Animal
/
Spleen
/
Bacterial Proteins
/
Lymphocyte Activation
/
Lipopolysaccharides
/
Immunization, Passive
/
Pasteurella multocida
/
Chemical Fractionation
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2000
Type:
Article
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