Effects of mixed leukocyte reaction, hydrocortisone and cyclosporine on expression of leukocyte adhesion molecules by endothelial and mesangial cells
Journal of Korean Medical Science
;
: 495-500, 1996.
Article
in English
| WPRIM
| ID: wpr-129317
ABSTRACT
We investigated the effects of mixed leukocyte reaction (MLR), hydrocortisone (HC) and cyclosporine A (CsA) on the expression of leukocyte adhesion molecules on the mesangial (MC) and endothelial cells (EnC). Cell surface enzyme immunoassay showed that INFnu, IL-1beta, or TNF alpha stimulated expression of ICAM-1, or VCAM-1 on MC after 24 hours. Flow cytometric analysis demonstrated that MLR supernatant induced a marked increase in mean fluorescence of or % of cells highly expressing intercellular adhesion molecule(ICAM)-1 or vascular cell adhesion molecule (VCAM)-1 on both cells after 24 hours (p<0.001). HC treatment(300 ng/ml) during MLR effectively inhibited MLR-induced upregulation of ICAM-1 and VCAM-1 on both cells (p<0.005). When MLR supernatant with HC was added to adhesion molecule assay, there was no inhibitory effect of HC on VCAM-1. CsA treatment (500 ng/ml) during MLR caused a modest decrease in upregulation of VCAM-1 on EnC (p<0.05), but had no effects on ICAM-1 on both cells. CsA directly decreased expression of VCAM-1 on MC. In conclusion, alloreactive lymphocytes and monocytes upregulate the expression of VCAM-1 and ICAM-1 on target cells probably by the mediation of cytokines. HC effectively prevents MLR-induced upregulation of VCAM-1 and ICAM-1. CsA does not increase the expression of VCAM-1 and ICAM-1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Hydrocortisone
/
Endothelium, Vascular
/
Monocytes
/
Cells, Cultured
/
Interleukin-1
/
Tumor Necrosis Factor-alpha
/
Cyclosporine
/
Lymphocyte Culture Test, Mixed
/
Intercellular Adhesion Molecule-1
/
Vascular Cell Adhesion Molecule-1
Limits:
Humans
Language:
English
Journal:
Journal of Korean Medical Science
Year:
1996
Type:
Article
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