Effect of L - tyrosine on Proliferation and Melanization in Cultured Normal Human Melanocytes / 대한피부과학회지
Korean Journal of Dermatology
;
: 25-30, 1996.
Article
in Korean
| WPRIM
| ID: wpr-129905
ABSTRACT
BACKGROUND:
The biosynthesis of melanin is initiated by the enzymatic oxidation of L-tyrosine to L-dopa by tyrosinase. Some precursors of melanin are cytotoxic, and melanoma cells are killed as a risk of exposare to excess tyrosine or dopa in the culture medium. However, there have been few observations of the effects of L-tyrosine on cultured normal human melanocyte.OBJECTIVE:
In order to investigate whether exogenous tyrosine induces cytotoxicity in cultured normal human melanocytes as in melanoma cells, we examined the effects of L-tyrosine on proliferation and melanization in normal human melanocytes.METHODS:
A melanocyte culture was produced with a modified TIC medium. L-tyrosine was added to the culture medium, 100, 200, 400, and 800uM. After 2 days of incubation, the proliferation was measured by methylthiazol tetrazolium(MTT) assay and sulforhodamine B(SRB) assay. The melanin contenis were also measured by the modified Whittaker's method.RESULTS:
On MTT assay, the proliferation of melanocytes had been stirnulated significantly (p< 0.05) in all L-tyrosine added groups. On SRB assay, the proliferation of melanocytes had heen stimulated significantly (p<005) in 200, 400, 800uM of L-tyrosine added groups. The melanin contents had increased in all L-tyrosine added groups, and had increased significantly (p<0.05) in 400uM of L-tyrosine added group.CONCLUSION:
L-tyrosine is not toxic to normal melanocytes, It stimulates the proliferation and melnization of cultured normal human melanocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tyrosine
/
Dihydroxyphenylalanine
/
Levodopa
/
Monophenol Monooxygenase
/
Tics
/
Melanins
/
Melanocytes
/
Melanoma
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
1996
Type:
Article
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