Gene Microarray Related with Apoptosis in Diabetic OLETF Keratocytes
Journal of the Korean Ophthalmological Society
;
: 818-827, 2006.
Article
in Korean
| WPRIM
| ID: wpr-130182
ABSTRACT
PURPOSE:
This study was to identified differentiated genes concerned with apoptosis that are up-regulated or down-regulated in OLETF corneal keratocytes stimulated with interleukin-1alpha.METHODS:
Otsuka Long Evans Tokushima Fatty (OLETF) corneal keratocytes were primarily cultured and treated with 20 ng/ml of IL-1a for 6 hours. Hybridization was carried out using Oligonucleotide microarray. A sample of normal keratocytes was labeled with Cy 3, and a diabetic keratocytes sample was labeled with Cy 5. RESULTS Diabetes showed 20 down-regulated genes and 24 up-regulated genes compared with normal keratocytes. Also, IL-1alpha-treated diabetic keratocytes expressed 31 down-regulated and 33 up-regulated genes. Compared with diabetic keratocytes, the newly expressed genes in OLETF treated with IL-1alpha were Bcl, Lam, Timp, Mmp, Socs, Bmp, Ccl, Lcn, C7, etc.CONCLUSIONS:
There were some genes related with apoptosis that expressed differently between normal and diabetic keratocytes of OLETF. Also, IL-1alpha may stimulate differing effects of apoptosis on diabetic corneal keratocytes. Studies analyzing the apoptotic significance of these differences identified in diabetic keratocytes are needed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apoptosis
/
Oligonucleotide Array Sequence Analysis
/
Diabetes Mellitus
/
Interleukin-1alpha
/
Corneal Keratocytes
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2006
Type:
Article
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