Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand
The Korean Journal of Parasitology
;
: 43-49, 2015.
Article
in English
| WPRIM
| ID: wpr-130570
ABSTRACT
The aim of the study was to explore the possible molecular markers of chloroquine resistance in Plasmodium vivax isolates in Thailand. A total of 30 P. vivax isolates were collected from a malaria endemic area along the Thai-Myanmar border in Mae Sot district of Thailand. Dried blood spot samples were collected for analysis of Pvmdr1 and Pvcrt-o polymorphisms. Blood samples (100 mul) were collected by finger-prick for in vitro chloroquine susceptibility testing by schizont maturation inhibition assay. Based on the cut-off IC50 of 100 nM, 19 (63.3%) isolates were classified as chloroquine resistant P. vivax isolates. Seven non-synonymous mutations and 2 synonymous were identified in Pvmdr1 gene. Y976F and F1076L mutations were detected in 7 (23.3%) and 16 isolates (53.3%), respectively. Analysis of Pvcrt-o gene revealed that all isolates were wild-type. Our results suggest that chloroquine resistance gene is now spreading in this area. Monitoring of chloroquine resistant molecular markers provide a useful tool for future control of P. vivax malaria.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Membrane Transport Proteins
/
Plasmodium vivax
/
Thailand
/
Drug Resistance
/
Protozoan Proteins
/
Chloroquine
/
Malaria, Vivax
/
Myanmar
/
Amino Acid Substitution
/
Inhibitory Concentration 50
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2015
Type:
Article
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