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Identification of Alternatively Spliced Forms of human OSCAR in Osteoclasts
Korean Journal of Bone Metabolism ; : 11-20, 2012.
Article in English | WPRIM | ID: wpr-130878
ABSTRACT

OBJECTIVES:

Osteoclasts are multinucleated giant cells which can resorb bone and differentiated from hematopoietic cells. We have previously reported murine osteoclast-associated receptor (OSCAR) may be an important bone-specific regulator of osteoclast differentiation. We have cloned soluble form of human OSCAR (hOSCAR) and examined the role of hOSCAR on osteoclast differentiation.

METHODS:

Osteoclast differentiation was induced by treatment with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor kappa B ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP) staining and pit formation were performed. Expression was measured by flow cytometry analysis, Northern and Western blot analysis.

RESULTS:

hOSCAR is expressed in osteoclast cells and involved in the differentiation of osteoclasts from peripheral blood mononuclear cells (PBMC). Two alternatively spliced forms (soluble hOSCAR [hOSCAR-S]) of hOSCAR were identified from osteoclasts complementary deoxyribonucleic acid (cDNA) library derived from PBMC. Putative transmembrane domain was not found in hOSCAR-S forms and it suggested that these forms might be secreted from osteoclast cells. These secreted forms of hOSCAR attenuated RANKL-induced osteoclast formation and bone resorption.

CONCLUSIONS:

Human osteoclasts express at least five different OSCAR messenger ribonucleic acid (mRNA) isoforms which could play different regulatory roles for differentiation. The secreted forms of hOSCAR might be a negative regulator of membrane-bounded forms of OSCAR.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Acid Phosphatase / DNA / RNA / Giant Cells / Blotting, Western / Macrophage Colony-Stimulating Factor / Clone Cells / Alternative Splicing / Protein Isoforms Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Korean Journal of Bone Metabolism Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Acid Phosphatase / DNA / RNA / Giant Cells / Blotting, Western / Macrophage Colony-Stimulating Factor / Clone Cells / Alternative Splicing / Protein Isoforms Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Korean Journal of Bone Metabolism Year: 2012 Type: Article