Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus
Journal of Veterinary Science
;
: 73-79, 2012.
Article
in English
| WPRIM
| ID: wpr-13089
ABSTRACT
Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After isopropyl-beta-D-thiogalactopyronoside induction, VP1Nd129 protein was shown to be successfully expressed in the E. coli. By performing an enzyme-linked immunoabsorbent assay using two coating antigens, purified VP1Nd129 and CAV-infected liver tissue lysate, E3 monoclonal antibody (mAb) was found to have higher reactivity against VP1 protein than the other positive clones according to the result of limiting dilution method from 64 clones. Using immunohistochemistry, the presence of the VP1-specific mAb, E3, was confirmed using CAV-infected liver and thymus tissues as positive-infected samples. Additionally, CAV particle purification was also performed using an immunoaffinity column containing E3 mAb. The monoclonal E3 mAb developed in this study will not only be very useful for detecting CAV infection and performing histopathology studies of infected chickens, but may also be used to purify CAV particles in the future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Poultry Diseases
/
Thymus Gland
/
Immunohistochemistry
/
Chickens
/
Specific Pathogen-Free Organisms
/
Circoviridae Infections
/
Chicken anemia virus
/
Capsid Proteins
/
Escherichia coli
/
Liver
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2012
Type:
Article
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