Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells
Journal of Veterinary Science
;
: 23-31, 2012.
Article
in English
| WPRIM
| ID: wpr-13096
ABSTRACT
Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-beta) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-beta, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-beta, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Leukocytes, Mononuclear
/
Dinoprostone
/
Cell Differentiation
/
Immunophenotyping
/
Transforming Growth Factor beta
/
Interferon-gamma
/
Interleukin-10
/
Hepatocyte Growth Factor
/
Coculture Techniques
Limits:
Female
/
Humans
/
Pregnancy
Language:
English
Journal:
Journal of Veterinary Science
Year:
2012
Type:
Article
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