Usefulness of Escherichia coli-expressed Recombinant VP6 Proteins of Group A Rotavirus in Serodiagosis of Rotavirus Infection / 대한소아소화기영양학회지
Korean Journal of Pediatric Gastroenterology and Nutrition
; : 134-145, 2010.
Article
in Ko
| WPRIM
| ID: wpr-130990
Responsible library:
WPRO
ABSTRACT
PURPOSE: The serologic diagnosis of rotaviral infections is not commonly used in clinical practice, but is used in seroepidemiologic studies. In this study, the usefulness of Escherichia coli-expressed recombinant VP6 proteins of group A rotavirus in the serodiagnosis of rotavirus infections by ELISA was evaluated. METHODS: The recombinant VP6 proteins of group A rotavirus expressed in E. coli Rosetta II strain were purified and identified. One hundred sera from 22 children (4 healthy neonates, 13 healthy children, and 5 immunocompromised children) who had serial sera samples prior to and after rotavirus infections were provided by the Gyeongsang National University Hospital, a member of the National Biobank of Korea. IgG, IgA, and IgM antibodies against rVP6 were analyzed by ELISA in all of the patients and Western blot analysis in 4 neonates. RESULTS: ELISA tests using rVP6 proteins of group A rotavirus as antigen revealed that IgG, IgA, and IgM antibodies increased after rotaviral infections in most neonates and healthy children. IgG antibodies also increased after rotaviral infections in most immunocompromised children without an adequate increase in IgM or IgA antibodies. Western blot analysis in four neonates revealed very early IgM antibody responses, even in the sera with low optical densities in ELISA tests. CONCLUSION: Our study showed that ELISA using rVP6 as an antigen is a valid diagnostic tool for seroepidemiologic studies of rotavirus infections and Western blot analysis is a sensitive test in detecting IgG, IgA, and and IgM antibodies in patients with rotavirus infections.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Rotavirus Infections
/
Sprains and Strains
/
Immunoglobulin A
/
Immunoglobulin G
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Proteins
/
Seroepidemiologic Studies
/
Blotting, Western
Type of study:
Diagnostic_studies
Limits:
Child
/
Humans
/
Newborn
Country/Region as subject:
Asia
Language:
Ko
Journal:
Korean Journal of Pediatric Gastroenterology and Nutrition
Year:
2010
Type:
Article