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In vitro and in vivo study of effects of fermented soybean product (chungkookjang) on NGF secretion ability and NGF receptor signaling pathway / 한국실험동물학회지
Laboratory Animal Research ; : 113-126, 2013.
Article in English | WPRIM | ID: wpr-13110
ABSTRACT
In order to investigate the effects of a fermented soybean product (Chungkookjang, CKJ) on nerve growth factor (NGF) metabolism, NGF secretion ability and its related signaling pathway were analyzed in B35 neuronal cells and the Tg2576 mouse model of Alzheimer's disease (AD). In B35 cells, the concentration of NGF significantly increased upon treatment with Taegwang (TG)-CKJ and Shinhwa (SH)-CKJ extracts compared with vehicle. Further, a significant increase in PC12 cell length as well as the phsophorylation levels of TrkA and Akt, which are members of a high affinity NGF receptor signaling pathway, were observed after treatment with TG-CKJ and SH-CKJ conditional medium (CM). On the other hand, there was no difference in activation of the NGF receptor p75NTR signaling pathway between vehicle and all CKJ treated groups. In Tg2576 mice showing early stage of AD, the concentrations of NGF in the serum and brain were reduced compared with those in Non-Tg mice. Treatment of Tg2576 mice with SH-CKJ, which contains high concentrations of total flavonoids and phenolic compounds, for 8 weeks dramatically recovered the NGF level to that of Non-Tg mice. Furthermore, the low phosphorylation levels of TrkA and Erk in the NGF receptor TrkA signaling pathway were rapidly recovered to those of Non-Tg mice after SH-CKJ treatment in vehicle treated Tg2576 mice, whereas the phosphorylation level of Akt was maintained at a constant level. These results suggest that CKJ may stimulate NGF secretion ability as well as the NGF receptor TrkA signaling pathway in PC12 cells and Tg2576 mice.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Soybeans / Flavonoids / Brain / PC12 Cells / Soybean Proteins / Phenol / Nerve Growth Factor / Receptor, Nerve Growth Factor / Alzheimer Disease Type of study: Prognostic study Limits: Animals Language: English Journal: Laboratory Animal Research Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Soybeans / Flavonoids / Brain / PC12 Cells / Soybean Proteins / Phenol / Nerve Growth Factor / Receptor, Nerve Growth Factor / Alzheimer Disease Type of study: Prognostic study Limits: Animals Language: English Journal: Laboratory Animal Research Year: 2013 Type: Article