A simple rat model of in situ reversible obstructive jaundice in situ reversible obstructive jaundice model
Annals of Surgical Treatment and Research
;
: 389-395, 2017.
Article
in English
| WPRIM
| ID: wpr-131730
ABSTRACT
PURPOSE:
To develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates.METHODS:
Rats were divided into 4 groups with 8 rats each the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed.RESULTS:
One rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group.CONCLUSION:
The present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bile Ducts
/
Body Weight
/
Choledochostomy
/
Drainage
/
Mortality
/
Common Bile Duct
/
Models, Animal
/
Jaundice, Obstructive
/
Inflammation
/
Laparotomy
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Annals of Surgical Treatment and Research
Year:
2017
Type:
Article
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