Matrix metalloproteinases in human gliomas: activation of matrix metalloproteinase-2 (MMP-2) may be correlated with membrane-type-1 matrix metalloproteinase (MT1-MMP) expression
Journal of Korean Medical Science
;
: 309-314, 2000.
Article
in English
| WPRIM
| ID: wpr-132614
ABSTRACT
To evaluate possible roles of matrix metalloproteinase (MMP)-1, -2, tissue inhibitor of metalloproteinase (TIMP)-1, -2 and membrane-type-1 matrix metalloproteinase (MT1-MMP) in invasion of human gliomas, expressions of these proteins were investigated in ten cases of human glioma and two meningioma tissues and eight human glioma cell lines. In gelatin zymography, MMP-2 activities of glioblastomas were higher than astrocytomas. The activated form of MMP-2 was seen in five of six cases of glioblastomas, but not in astrocytomas. MMP-9 activity was detected in all cases of malignant astrocytomas but the reactivity of MMP-9 was weaker than that of MMP-2. MT1-MMP mRNA expression in glioblastomas was higher than that in astrocytomas. Five cases of glioblastomas with activated form of MMP-2 had MT1-MMP expressions. In vitro, human glioma cell lines with high expression of MT1-MMP also showed high MMP-2 activity. TIMP-1 transcripts were constitutively present in almost all glioma tissues and cell lines, whereas TIMP-2 mRNA were weak especially in malignant gliomas. Imbalance of TIMP-2/MMP-2 was observed using immunoprecipitation analysis in a glioma cell line. High expression of MMP-2 and MT1-MMP is possibly involved in invasiveness of malignant glioma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papio
/
Brain
/
Brain Neoplasms
/
Metalloendopeptidases
/
Tumor Cells, Cultured
/
Gene Expression Regulation, Enzymologic
/
Blotting, Northern
/
Tissue Inhibitor of Metalloproteinase-1
/
Tissue Inhibitor of Metalloproteinase-2
/
Matrix Metalloproteinase 2
Limits:
Humans
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2000
Type:
Article
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