Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells
Journal of Korean Medical Science
;
: 273-278, 2000.
Article
in English
| WPRIM
| ID: wpr-132632
ABSTRACT
Atherosclerosis is an inflammatory disease. Platelet-endothelium interaction plays an important role in the pathophysiology of atherogenesis. We investigated the role of activated platelets for secretion of interleukin (IL)-1beta, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha and expression of intercellular adhesion molecule (ICAM)-1 on endothelial cells. Human umbilical vein endothelial cells (HUVEC) were incubated with non-stimulated or ADP-activated platelets for 6 hr. Secretion of interleukin (IL)-1beta, MCP-1 and MIP-1alpha and surface expression of ICAM-1 were measured by ELISA and flow cytometry. In the presence of activated platelets, the secretion of IL-1beta, MCP-1, and MIP-1alpha and surface expression of ICAM-1 were significantly increased compared with non-activated platelets. The present study shows that activated platelets may contribute to expression of various inflammatory mediators on endothelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood Platelets
/
Endothelium, Vascular
/
Platelet Activation
/
Cells, Cultured
/
Interleukin-1
/
Intercellular Adhesion Molecule-1
/
Chemokine CCL2
/
Macrophage Inflammatory Proteins
Limits:
Humans
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2000
Type:
Article
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