Age - Related Changes of Glycosaminoglycan in Senescence - Accelerated Mouse ( SAM ) Skin / 대한피부과학회지
Korean Journal of Dermatology
;
: 631-639, 1994.
Article
in Korean
| WPRIM
| ID: wpr-132736
ABSTRACT
BACKGROUND:
The various manifestations of intrinsic cutaneous aging may reflect the age-related changes of dermal ground aubstances, the important components of the dermis.OBJECTIVE:
This study was undertaken to observe whether there is any age-relsted change, quantitatively and qualitatively, in dermal glycosaminoglycan(GAG)METHODS:
The Senescence-A.ccelerated-Mice(SAM) strain "Prone" to develop senescence(P/8), aged 4 days(P-Y a group, n=5) and 4 months old (P-0 group, n=5) were used, with SAM strain Resistance to senescence (R/1), aged 4 montha old (R-0 group, n=5). The whole skin of SAM was incubated in 0.1 sodium phosphate buffer(NaPB), 2M guanidine-HC1, and 4M guanidine-HCl, sequencially, for the extractiop of GAG. The amount of GAG was measured by using alcian blue and by methylene blue metachrometic assay. Uronic acid(UA) was estimate l employing carbazole reaction. Extracted skin protein profiles were analyed by SDS-PAGE.RESULTS:
1. The total contents of GAG per wet weight of skin, as measured uairq, alcian blue, was highter in R-0 group than R-Y and P-Y group, which is statistically significant. 2. The methylene blue metaehromatic assay yielded highter absorbance values in 2M guanidine-HCl extract than NaPB extracts. 3. The total contents of UA decreased with aging in R strains, buttriking increase was noted in the P strain. 4. On SDS-PAGE, the protein profiles of NaPB extracts showed similarity to serum protein. 125 kDa protein band was noted only in guanidine-HCl extracts. 37.40kDa protein bands were appeared in 2M, 4M guanicline-HCl extract of R-0 group. But. there was no significant difference in both strains.CONCLUSION:
These findings suggest that macromolecules, such as GAG, is one of the target molecules of the cutaneous eging process, and these change muy be related to the age-related changes of dermal water content.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Sodium
/
Aging
/
Epidermolysis Bullosa Acquisita
/
Pemphigoid, Bullous
/
Dermis
/
Alcian Blue
/
Electrophoresis, Polyacrylamide Gel
/
Methylene Blue
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
1994
Type:
Article
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