Megakaryocyte Colony Formation of Fetal Liver Cells
Journal of the Korean Pediatric Society
;
: 247-255, 2002.
Article
in Korean
| WPRIM
| ID: wpr-13333
ABSTRACT
PURPOSE:
This study was undertaken to obtain basic data about the megakaryocyte colony formation of fetal liver cells by using immunocytochemical staining and ex vivo culture with growth factors.METHODS:
The mononuclear cells were isolated from fetal liver and bone marrow with idiopathic thrombocytopenic purpura(ITP) and pancytopenia. These mononuclear cells were cultured in MegaCultTM-C(Stem Cell Tech, Canada) media in the presence of growth factors and CFU-Megakaryocyte(CFU-Mk) colonies were counted on day 12. The expansion of CD34+ and CD41+ cell was analyzed by flow cytometry after 5 days incubation using flask culture.RESULTS:
The numbers of CFU-Mk colonies of mononuclear cells obtained from fetal liver in the 11th week gestational age were more than those in the 19th week specimens; growth factors could not enhance the colony expansion in all cases. Total numbers of CFU-Mk colony of fetal liver cells were higher than bone marrow from ITP or pancytopenia groups. The numbers of pure or large CFU-Mk colonies of fetal liver cells were also higher than bone marrow specimens. The rate of CD34+ cell expression of fetal liver was increased after flask culture and the enhancement effect of epression was seen only in cases which added thrombopoietin. The rate of CD41+ cell expression of fetal liver was increased after incubation, but the enhancement effect of growth factors was unclear.CONCLUSION:
This study revealed good results about the megakaryocyte colony assay of fetal liver mononuclear cells using MegaCultTM-C media. This study suggests that the fetal liver could be a good source of megakaryocytic progenitor cells for clinical application in hematopoietic stem cell transplantation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancytopenia
/
Stem Cells
/
Thrombopoietin
/
Bone Marrow
/
Hematopoietic Stem Cells
/
Megakaryocytes
/
Gestational Age
/
Hematopoietic Stem Cell Transplantation
/
Intercellular Signaling Peptides and Proteins
/
Flow Cytometry
Language:
Korean
Journal:
Journal of the Korean Pediatric Society
Year:
2002
Type:
Article
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