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Clinical Evaluation of Rapid Diagnostic Test Kit Using the Polysaccharide as a Genus-Specific Diagnostic Antigen for Leptospirosis in Korea, Bulgaria, and Argentina
Journal of Korean Medical Science ; : 183-189, 2016.
Article in English | WPRIM | ID: wpr-133739
ABSTRACT
Leptospirosis, a zoonotic disease that is caused by many serovars which are more than 200 in the world, is an emerging worldwide disease. Accurate and rapid diagnostic tests for leptospirosis are a critical step to diagnose the disease. There are some commercial kits available for diagnosis of leptospirosis, but the obscurity of a species- or genus-specific antigen of pathogenic Leptospira interrogans causes the reduced sensitivity and specificity. In this study, the polysaccharide derived from lipopolysaccharide (LPS) of nonpathogenic Leptospira biflexa serovar patoc was prepared, and the antigenicity was confirmed by immunoblot and enzyme linked immunosorbent assay (ELISA). The performance of the rapid diagnostic test (RDT) kit using the polysaccharide as a diagnostic antigen was evaluated in Korea, Bulgaria and Argentina. The sensitivity was 93.9%, 100%, and 81.0% and the specificity was 97.9%, 100%, and 95.4% in Korea (which is a rare region occurring with 2 serovars mostly), Bulgaria (epidemic region with 3 serovars chiefly) and Argentina (endemic region with 19 serovars mainly) respectively. These results indicate that this RDT is applicable for global diagnosis of leptospirosis. This rapid and effective diagnosis will be helpful for diagnosis and manage of leptospirosis to use and the polysaccharide of Leptospira may be called as genus specific antigen for diagnosis.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Argentina / Polysaccharides / Reagent Kits, Diagnostic / Bulgaria / Enzyme-Linked Immunosorbent Assay / Sensitivity and Specificity / Republic of Korea / Leptospira / Leptospira interrogans / Leptospirosis Type of study: Diagnostic study Limits: Female / Humans / Male Country/Region as subject: South America / Argentina / Asia / Europa Language: English Journal: Journal of Korean Medical Science Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Argentina / Polysaccharides / Reagent Kits, Diagnostic / Bulgaria / Enzyme-Linked Immunosorbent Assay / Sensitivity and Specificity / Republic of Korea / Leptospira / Leptospira interrogans / Leptospirosis Type of study: Diagnostic study Limits: Female / Humans / Male Country/Region as subject: South America / Argentina / Asia / Europa Language: English Journal: Journal of Korean Medical Science Year: 2016 Type: Article