Alloimmune and Skin Allograft Responses in 4-1BB (CD137)-deficient Mice
Immune Network
;
: 133-136, 2002.
Article
in English
| WPRIM
| ID: wpr-134615
ABSTRACT
BACKGROUND:
The costimulatory molecule 4-1BB, a member of nerve growth factor receptor/tumor necrosis factor (NGFR/TNFR) super family, is involved in cell survival and death.METHODS:
In this study, female C57BL/6 (H-2(b)) mice were used as a recipient, and DBA/2 (H-2(d)) as a donor to assess a mixed lymphocyte reaction (MLR) and CTL response in vitro, and skin graft survival. IL-2, IFN level was measured by ELISA.RESULTS:
Mixed lymphocyte reaction (MLR) analysis showed that 4-1BB- deficient responder cells showed enhanced cellular proliferation over littermate controls. In contrast, IL-2 production was diminished only in 4-1BB knockout cultures. The IFN expression, on the other hand, was comparable between the groups. When female C57BL/6 (H-2(b)) mice were grafted with the trunk skin of DBA/2 (H-2d) mice, the in vivo tissue destruction of 4-1BB-deficient mice was not distinct from the normal littermates.CONCLUSION:
These data suggest that 4-1BB is critical for the induction of alloreactive responses in vitro but 4-1BB alone could not change the course of skin rejection in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Tissue Donors
/
Enzyme-Linked Immunosorbent Assay
/
Cell Survival
/
Interleukin-2
/
Lymphocyte Culture Test, Mixed
/
Transplants
/
Nerve Growth Factor
/
Cell Proliferation
/
Allografts
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Immune Network
Year:
2002
Type:
Article
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