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Duration and Magnitude of Extracellular Signal-Regulated Protein Kinase Phosphorylation Determine Adipogenesis or Osteogenesis in Human Bone Marrow-Derived Stem Cells
Yonsei Medical Journal ; : 165-172, 2011.
Article in English | WPRIM | ID: wpr-136372
ABSTRACT

PURPOSE:

Imbalances between osteogenic and adipogenic differentiation leads to diseases such as osteoporosis. The aim of our study was to demonstrate the differences in extracellular signal-regulated kinase (ERK) phosphorylation during both adipogenesis and osteogenesis of human bone marrow-derived stem cells (BMSCs). MATERIALS AND

METHODS:

Using troglitazone, GW9662 and U0126, we investigated their role in hBMSC differentiation to adipogenic and osteogenic fates.

RESULTS:

ERK1/2 inhibition by U0126 suppressed proliferator-activated receptor (PPAR)gamma expression and lipid accumulation, while it decreased the mRNA expression of adipogenic genes (lipoprotein lipase, PPARgamma, and adipocyte protein) and osteogenic genes (type I collagen and osteopontin). ERK phosphorylation was transient and decreased during adipogenesis, whereas it occurred steadily during osteogenesis. Troglitazone, a PPARgamma agonist, induced adipogenesis by inhibiting ERK phosphorylation even in an osteogenic medium, suggesting that ERK signaling needs to be shut off in order to proceed with adipose cell commitment. Cell proliferation was greatly increased in osteogenesis but was not changed during adipogenesis, indicating that ERK might play different roles in cellular proliferation and differentiation between the two committed cell types.

CONCLUSION:

The duration and magnitude of ERK activation might be a crucial factor for the balance between adipogenesis and osteogenesis in human bone marrow-derived stem cells.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Phosphorylation / Stem Cells / Butadienes / Bone Marrow Cells / Cell Differentiation / Cells, Cultured / Chromans / Reverse Transcriptase Polymerase Chain Reaction / Thiazolidinediones Limits: Adult / Female / Humans / Male Language: English Journal: Yonsei Medical Journal Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Phosphorylation / Stem Cells / Butadienes / Bone Marrow Cells / Cell Differentiation / Cells, Cultured / Chromans / Reverse Transcriptase Polymerase Chain Reaction / Thiazolidinediones Limits: Adult / Female / Humans / Male Language: English Journal: Yonsei Medical Journal Year: 2011 Type: Article