Polymorphisms of COTL1 gene identified by proteomic approach and their association with autoimmune disorders
Experimental & Molecular Medicine
;
: 354-361, 2009.
Article
in English
| WPRIM
| ID: wpr-136581
ABSTRACT
To select candidate genes, we attempted to comparative analysis of protein levels between rheumatoid arthritis (RA) patients and healthy controls by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF-MS). We identified 17 proteins that showed up- or down-regulated spots in RA patients. We found that coactosin-like1 (COTL1) were highly expressed in RA patients compared with healthy controls. We performed a case-control study to determine whether the COTL1 gene polymorphisms were associated with RA and systemic lupus erythematosus (SLE). The genotype frequency of c.-1124G>T and the allelic frequency of c.484G>A in RA patients, and the genotype frequency of c.484G>A in SLE patients were significantly different from healthy controls (P = 0.009, 0.027, and 0.025, respectively). We also investigated the correlation with the levels of rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCP) antibody in RA patients, and anti-nuclear antibodies (ANA) in SLE patients. The c.484G>A polymorphism in RA patients has significant association with the levels of anti-CCP antibody (P = 0.03). Our findings demonstrated that c.-1124G>T and c.484G>A polymorphisms of the COTL1 gene might be associated with the genetic susceptibility of autoimmune disorders.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Arthritis, Rheumatoid
/
Autoimmune Diseases
/
Electrophoresis, Gel, Two-Dimensional
/
Case-Control Studies
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Proteome
/
Proteomics
/
Genotype
/
Lupus Erythematosus, Systemic
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2009
Type:
Article
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