Prevalence of CTX-M Extended-spectrum Beta-lactamases in Clinical Isolates of Enterobacteriaceae in Korea
Journal of Bacteriology and Virology
;
: 303-310, 2004.
Article
in English
| WPRIM
| ID: wpr-138069
ABSTRACT
The evolution and dissemination of extended-spectrum beta-lactamases (ESBL) have compromised the clinical use of third-generation cephalosporins worldwide. Although most ESBLs belong to the TEM and SHV beta-lactamase families, the members of CTX-M, a novel ESBL family, are increasing worldwide in Gram-negative bacteria. We examined the prevalence of CTX-M ESBL in clinical isolates of the family Enterobacteriaceae collected from three university hospitals located in three different cities in Korea. Among a total of 603 isolates collected, 163 isolates (27.0%) revealed > or =2 microgram/ ml of MIC against cefotaxime, and 93 isolates (15.4%) produced ESBL confirmed by the double disk synergy test. Among 93 ESBL-producing isolates, blaCTX-M genes were detected in 41 isolates by PCR method and they included 1 isolate of C. freundii, 3 of E. aerogenes, 2 of E. cloacae, 17 of E. coli, 9 of K. pneumoniae, and 9 of S. marcescens. Thus, the overall prevalence of CTX-M ESBL-producing isolates among the family Enterobacteriaceae was 6.8% (41 of 603 isolates) and the proportion of CTX-M-producers among the ESBL-producing isolates was 44.1% (41 of 93 isolates). Further determination of the blaCTX-M subtype by nucleotide sequencing revealed blaCTX-M-3 in 17, blaCTX-M-15 in 11, blaCTX-M-14 in 9, and blaCTX-M-9 in 4 isolates. To our knowledge, this is the first report on the dissemination of CTX-M ESBL among clinical isolates of the family Enterobacteriaceae in Korea.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Beta-Lactamases
/
Cefotaxime
/
Cephalosporins
/
Polymerase Chain Reaction
/
Prevalence
/
Cloaca
/
Enterobacteriaceae
/
Gram-Negative Bacteria
/
Hospitals, University
Type of study:
Prevalence study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Bacteriology and Virology
Year:
2004
Type:
Article
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