Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
Korean Journal of Veterinary Research
;
: 189-192, 2016.
Article
in English
| WPRIM
| ID: wpr-13821
ABSTRACT
The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 log₂ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bronchitis
/
Enzyme-Linked Immunosorbent Assay
/
Hemagglutination Inhibition Tests
/
Neutralization Tests
/
Infectious bronchitis virus
/
Vaccine Potency
/
Hemagglutination
Language:
English
Journal:
Korean Journal of Veterinary Research
Year:
2016
Type:
Article
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