IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
The Korean Journal of Parasitology
;
: 99-102, 2012.
Article
in English
| WPRIM
| ID: wpr-138589
ABSTRACT
Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parasitology
/
Immunoglobulin G
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Protozoan
/
Toxoplasmosis
/
Fluorescent Antibody Technique, Indirect
/
Clinical Laboratory Techniques
/
Iran
/
Antibody Affinity
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2012
Type:
Article
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