Species Identification Using Cytochrome B Gene / 대한법의학회지
Korean Journal of Legal Medicine
;
: 34-39, 2001.
Article
in Korean
| WPRIM
| ID: wpr-140120
ABSTRACT
The feasibility of species identification using sequence analysis of the cytochrome B (Cyt B) gene in mitochondrial DNA was investigated. DNA was extracted from nine different animals that could be easily met in our surroundings and Cyt B gene was amplified. Direct sequencing results for the amplified PCR products were compared with each other. Human was also included. Nucleotide sequence of the Cyt B gene for each animals was also compared with the previously known ones registered in nucleotide databases, Genebank. The inter-species sequence variation was high as the percent similarity of each sequences ranged 64.6-83.5%. Compared to this the percent similarity of sequences obtained here were high when compared to the sequences of the same species registered in the database showing relatively low intra-species variation. This data shows that the nucleotide sequences of Cyt B gene in a certain biological materials can be identified at species level. The applicability of this method to the forensic field is also demonstrated by performing a casework; determination of the origin for the placentae which were commercial available as "invogorant". Points about the use of Cyt B gene in forensic field was also reviewed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
DNA
/
DNA, Mitochondrial
/
Base Sequence
/
Polymerase Chain Reaction
/
Sequence Analysis
/
Cytochromes
/
Cytochromes b
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Legal Medicine
Year:
2001
Type:
Article
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