Comparison between L and E gene amplification analytical methods for human papillomavirus typing / 부인종양
Journal of Gynecologic Oncology
;
: 251-255, 2008.
Article
in English
| WPRIM
| ID: wpr-140247
ABSTRACT
OBJECTIVE:
L and E6/E7 gene amplification analyses were compared to identify human papillomavirus (HPV) infection and verify the HPV type, with the intent to minimize HPV typing errors.METHODS:
L1 gene verified HPV typing was accomplished via polymerase chain reaction (PCR) and membrane assays. Verification of HPV typing via E6/E7 genes was accomplished through nested multiplexed PCR. The results from 104 samples were compared.RESULTS:
The rates of accordance and difference were 35% and 65%, respectively. For 29% of the analyses, nested multiplexed PCR was more diversified than the membrane assay.CONCLUSION:
HPV can be classified into low-risk HPV and high-risk HPV groups. In parallel amplifications of the L and E genes is more efficient for accurate diagnosis in light of the different symptoms and attendant precautions of the risk groups.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gene Amplification
/
Polymerase Chain Reaction
/
Light
/
Membranes
Limits:
Humans
Language:
English
Journal:
Journal of Gynecologic Oncology
Year:
2008
Type:
Article
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