Anti-apoptotic effect of fermented Citrus sunki peel extract on chemical hypoxia-induced neuronal injury / 한국영양학회지
Journal of Nutrition and Health
;
: 451-456, 2015.
Article
in Korean
| WPRIM
| ID: wpr-14025
ABSTRACT
PURPOSE:
Neuronal apoptotic events induced by aging and hypoxic/ischemic conditions is an important risk factor in neurodegenerative diseases such as ischemia stroke and Alzheimer's disease. The peel of Citrus sunki Hort. ex Tanaka has long been used as a traditional medicine, based on multiple biological activities including anti-oxidant, anti-inflammation, and anti-obesity. In the current study, we examined the actions of fermented C. sunki peel extract against cobalt chloride (CoCl2)-mediated hypoxic death in human neuroblastoma SH-SY5Y cells.METHODS:
Cell viability was measured by trypan blue exclusion. Expression of apoptosis related proteins and release of cytochrome c were detected by western blot. Production of intracellular reactive oxygen species (ROS) and apoptotic morphology were examined using 2',7'-dichlorofluorescin diacetate (DCF-DA) and 4',6-diamidino-2-phenylindole (DAPI) staining.RESULTS:
Exposure to CoCl2, a well-known mimetic agent of hypoxic/ischemic condition, resulted in neuronal cell death via caspase-3 dependent pathway. Extract of fermented C. sunki peel significantly rescued the CoCl2-induced neuronal toxicity with the cell viability and appearance of apoptotic morphology. Cytoprotection with fermented C. sunki peel extract was associated with a decrease in activities of caspase-3 and cleavage of poly (ADP ribose) polymerase (PARP). In addition, increase in the intracellular ROS and release of cytochrome c from mitochondria to the cytosol were inhibited by treatment with extract of fermented C. sunki peel.CONCLUSION:
Based on these data, fermented C. sunki peel extract might have a protective effect against CoCl2-induced neuronal injury partly through generation of ROS and effectors involved in mitochondrial mediated apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trypan Blue
/
Aging
/
Cell Survival
/
Blotting, Western
/
Risk Factors
/
Citrus
/
Cell Death
/
Reactive Oxygen Species
/
Cobalt
/
Apoptosis
Type of study:
Etiology study
/
Risk factors
Limits:
Humans
Language:
Korean
Journal:
Journal of Nutrition and Health
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS