Hypermethylation of E-cadherin in endometrial carcinoma / 부인종양
Journal of Gynecologic Oncology
;
: 241-245, 2008.
Article
in English
| WPRIM
| ID: wpr-140251
ABSTRACT
OBJECTIVE:
Hypermethylation of CpG island is a common mechanism for the inactivation of tumor suppressor genes. Hypermethylation of the E-cadherin promoter region has been rarely studied in endometrial carcinoma of Korean women. The purpose of this study is to investigate methylation status of E-cadherin promoter region in endometrial carcinomas and endometrial hyperplasias, and analyze the correlation with clinicopathologic variables in endometrial carcinomas.METHODS:
We examined the methylation status of the E-cadherin promoter region using methylation specific polymerase chain reaction and immunohistochemical expression (IHC) of E-cadherin in 30 endometrioid endometrial carcinomas and 20 endometrial hyperplasias, and correlated these results with various clinicopathological factors of endometrial carcinomas.RESULTS:
Decreased expression of E-cadherin was detected in 13 of 30 (43.3%) endometrial carcinomas and in 1 of 20 (5%) endometrial hyperplasias (p=0.009). Promoter hypermethylation was detected in 12 of 30 (40%) endometrial carcinomas and 2 of 20 (10%) endometrial hyperplasias (p=0.015). Methylation status did not have a significant influence on the tumor grade and lymph node metastasis. However, the hypermethylation rate was significantly higher in stage above Ic (p=0.025). Decreased expression of E-cadherin was associated with tumor grade, tumor stage, and lymph node metastasis in endometrial carcinomas (p=0.01, p=0.02, p=0.03). There was no correlation between DNA hypermethylation and decreased expression of E-cadherin in endometrial carcinomas (p>0.05).CONCLUSION:
These results indicate that hypermethylation of E-cadherin promoter region is a frequent event in endometrial carcinoma, which may play an important role in the progression of carcinogenesis. Also, the promoter methylation of E-cadherin in endometrial carcinoma was found to be significantly associated with higher stage above Ic.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Cadherins
/
Polymerase Chain Reaction
/
Genes, Tumor Suppressor
/
Promoter Regions, Genetic
/
Endometrial Neoplasms
/
CpG Islands
/
Endometrial Hyperplasia
/
Lymph Nodes
/
Methylation
Limits:
Female
/
Humans
Language:
English
Journal:
Journal of Gynecologic Oncology
Year:
2008
Type:
Article
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