Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell
Immune Network
;
: 1-5, 2006.
Article
in English
| WPRIM
| ID: wpr-140315
ABSTRACT
BACKGROUND:
B cell subset has been divided into B-1 cells and B-2 cells. B-1 cells are found most prominently in the peritoneal cavity, as well as constituting a small proportion of splenic B cells and they are larger and less dense than B-2 cells in morphology. This study was designed to compare the differences in their proliferation and differentiation between B-1 and B-2 cell.METHODS:
We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. Secreted IgM was measured by enzyme-linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Cell cycle analysis by propidium iodide was performed. p21 expression was assessed by real time PCR.RESULTS:
Cell proliferation and cell cycle progression in B-1 and B-2 cells, which did not occur in the absence of LPS, required LPS stimulation. After LPS stimulation, B-1 and B-2 cells were shifted to S and G2/M phases. p21 expression by resting B-1 cells was higher than that of resting B-2 cells.CONCLUSION:
B-1 cells differ from conventional B-2 cells in proliferation, differentiation and cell cycle.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peritoneal Cavity
/
Propidium
/
Spleen
/
Ascitic Fluid
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
B-Lymphocytes
/
Cell Cycle
/
S Phase
/
Cell Proliferation
Limits:
Animals
Language:
English
Journal:
Immune Network
Year:
2006
Type:
Article
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