Comparison of Apoptosis and Bcl-2 Expression in Normal Pregnancy, Pregnancy Induced Hypertension and Intrauterine Growth Restriction / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 278-286, 2004.
Article
in Korean
| WPRIM
| ID: wpr-140721
ABSTRACT
OBJECTIVE:
The purpose of this study was to investigate the incidence of apoptosis and expression of bcl-2 in the placenta of normal pregnancy, Pregnancy Induced Hypertension, and Intrauterine Growth Restriction.METHODS:
Placenta samples were collected from 15 cases of normal full-term pregnancies, 15 cases of second trimester pregnancies, 17 cases of Pregnancy Induced Hypertension, and 13 cases of Intrauterine Growth Restriction. Hematoxylin and eosin staining and TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate marker nick end-labeling) staining were used to quantify the incidence of apoptosis and the electron microscopy was used to confirm it. Expression of bcl-2 was confirmed by using immunohistochemical stain in relation to apoptosis.RESULTS:
1. In TUNEL staining, quantification of apoptosis was 1.05 in 2nd trimester (n=15), 3.65 in pregnancy induced hypertensive pregnancy (n=17), 2.92 in intrauterine growth restrictive pregnancy (n=13) and 1.93 in normal full-term pregnancy (n=15). The incidence of apoptosis was significantly higher in placental tissues from full-term pregnancies than second trimester pregnancies (p0.05, t test), and between intrauterine growth restriction and normal full-term pregnancy (p>0.05, Mann-Whitney U test).CONCLUSION:
These data suggest that apoptosis increases with gestational age, and in pathophysiologic states such as pregnancy induced hypertension and intrauterine growth restriction, and that bcl-2 expression is lower with gestational age.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Pregnancy Trimester, Second
/
Microscopy, Electron
/
Incidence
/
Gestational Age
/
Apoptosis
/
Eosine Yellowish-(YS)
/
In Situ Nick-End Labeling
/
Hypertension, Pregnancy-Induced
/
Deoxyuridine
Type of study:
Incidence study
/
Prognostic study
Limits:
Female
/
Humans
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2004
Type:
Article
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