Detection of Glycoproteins (B and D) and Ttymidine Kinase Genes of Herpes simplex virus Type 2 Strain G
Journal of the Korean Society of Virology
; : 99-105, 1999.
Article
in En
| WPRIM
| ID: wpr-142038
Responsible library:
WPRO
ABSTRACT
BamHI restriction patters and genomic library of Herpes simplex virus type 2 (HSV-2) stram G were constructed, and locations of the glycoproteins gB and gD, and it genes on the fragments were detected by Southern blot analysis. HISV-2 genomic DNAs were cleaved into twenty-seven fragments by BamHI enzyme in the range of 0.72 to 15.08 (total 150.44 kb), which were cloned into the BamHI site of pBluescript SK(+) to construct genome library of the HSV-2. The library was named by the order of the fragment size from smallest one to largest one. HSV-2 glycoprotein gD gene was located in PHLA2-21 and PHLA2-22 recombinant plasmids, gB gene in PHLA2-24 plasmic, and it gene in PHLA2-11 clone by Southern blot analysis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phosphotransferases
/
Plasmids
/
DNA
/
Glycoproteins
/
Genomic Library
/
Blotting, Southern
/
Clone Cells
/
Simplexvirus
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Herpesvirus 2, Human
/
Herpes Simplex
Type of study:
Diagnostic_studies
Language:
En
Journal:
Journal of the Korean Society of Virology
Year:
1999
Type:
Article