Effect of Bimatoprost on the Permeability of Trabecular Meshwork Cell Monolayer
Journal of the Korean Ophthalmological Society
;
: 586-591, 2015.
Article
in Korean
| WPRIM
| ID: wpr-14241
ABSTRACT
PURPOSE:
To investigate the effects of bimatoprost on the permeability of cultured human trabecular meshwork cells (HTMC) monolayer.METHODS:
HTMCs were cultured until confluency in the inner Transwell chamber and then exposed to benzalkonium chloride, brimonidine, latanoprost or bimatoprost for 1 week. Carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer after 2 hours in the outer chamber. Cellular viability was assessed using the MTT assay.RESULTS:
Each drug diluted at 1/1000X did not affect the cellular survival (p > 0.05). Brimonidine, latanoprost and bimatoprost did not affect the carboxyfluorescein permeability through the HTMC monolayer (p > 0.05). The carboxyfluorescein permeability was not different between latanoptost and bimatoprost after 1 week of exposure (p > 0.05).CONCLUSIONS:
Bimatoprost, a drug known to increase trabecular outflow, does not affect the carboxyfluorescein permeability through the HTMC monolayer. Thus, the effect on the trabecular outflow of bimatoprost may not be significant.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Trabecular Meshwork
/
Benzalkonium Compounds
/
Bimatoprost
/
Brimonidine Tartrate
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2015
Type:
Article
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