Troglitazone Enhances the Apoptotic Response of DLD-1 Colon Cancer Cells to Photodynamic Therapy
Yonsei Medical Journal
;
: 1494-1499, 2016.
Article
in English
| WPRIM
| ID: wpr-143156
ABSTRACT
PURPOSE:
The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. MATERIALS ANDMETHODS:
The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses.RESULTS:
Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3.CONCLUSION:
Conclusion:
These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Photochemotherapy
/
Propidium
/
Cell Survival
/
Blotting, Western
/
Cell Death
/
Apoptosis
/
Colon
/
Colonic Neoplasms
/
Caspase 3
/
Necrosis
Language:
English
Journal:
Yonsei Medical Journal
Year:
2016
Type:
Article
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