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Troglitazone Enhances the Apoptotic Response of DLD-1 Colon Cancer Cells to Photodynamic Therapy
Yonsei Medical Journal ; : 1494-1499, 2016.
Article in English | WPRIM | ID: wpr-143156
ABSTRACT

PURPOSE:

The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. MATERIALS AND

METHODS:

The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses.

RESULTS:

Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3.

CONCLUSION:

Conclusion:

These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Photochemotherapy / Propidium / Cell Survival / Blotting, Western / Cell Death / Apoptosis / Colon / Colonic Neoplasms / Caspase 3 / Necrosis Language: English Journal: Yonsei Medical Journal Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Photochemotherapy / Propidium / Cell Survival / Blotting, Western / Cell Death / Apoptosis / Colon / Colonic Neoplasms / Caspase 3 / Necrosis Language: English Journal: Yonsei Medical Journal Year: 2016 Type: Article