Effects of excessive dietary methionine on oxidative stress and dyslipidemia in chronic ethanol-treated rats
Nutrition Research and Practice
;
: 144-149, 2015.
Article
in English
| WPRIM
| ID: wpr-143314
ABSTRACT
BACKGROUND/OBJECTIVE:
The aim of this study was to examine the effect of high dietary methionine (Met) consumption on plasma and hepatic oxidative stress and dyslipidemia in chronic ethanol fed rats. MATERIALS/METHODS:
Male Wistar rats were fed control or ethanol-containing liquid diets supplemented without (E group) or with DL-Met at 0.6% (EM1 group) or 0.8% (EM2 group) for five weeks. Plasma aminothiols, lipids, malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase were measured. Hepatic folate, S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) were measured.RESULTS:
DL-Met supplementation was found to increase plasma levels of homocysteine (Hcy), triglyceride (TG), total cholesterol (TC), and MDA compared to rats fed ethanol alone and decrease plasma ALT. However, DL-Met supplementation did not significantly change plasma levels of HDL-cholesterol, cysteine, cysteinylglycine, and glutathione. In addition, DL-Met supplementation increased hepatic levels of folate, SAM, SAH, and SAMSAH ratio. Our data showed that DL-Met supplementation can increase plasma oxidative stress and atherogenic effects by elevating plasma Hcy, TG, and TC in ethanol-fed rats.CONCLUSION:
The present results demonstrate that Met supplementation increases plasma oxidative stress and atherogenic effects by inducing dyslipidemia and hyperhomocysteinemia in ethanol-fed rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasma
/
Aspartate Aminotransferases
/
S-Adenosylhomocysteine
/
S-Adenosylmethionine
/
Triglycerides
/
Cholesterol
/
Rats, Wistar
/
Oxidative Stress
/
Hyperhomocysteinemia
/
Cysteine
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Nutrition Research and Practice
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS